Search results for "Nonsense polarity"

Nonsense polarity, RNA processing and decay in phage f1.

2011

Nonsense polarity in most cases depends on activation of cryptic transcription terminators. We found that the strong polar effect observed in the nonsense polar mutant R4 of phage f1, mapping in the 5’ proximal region of gene III, instead depends on enhanced instability of mutant mRNAs, whose pattern can be restored by reduction of RNase E activity. rne -(ts) E. coli strains allowed to explore the mechanisms underlying f1 mRNA processing and degradation. The major gene III species, a 1.8 Kb long molecule, appeared to be a secondary transcript, whose decay is modulated by a REP, located at its 3' end. The RNA pool of a mutagenized phage unable to form that structure, lacks completely that tr…